A Duplex Real-Time PCR Assay for the Simultaneous Detection of Porcine Circovirus 2 and Circovirus 3
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چکیده
منابع مشابه
Development of TaqMan Duplex Real-time PCR for Simultaneous Detection of Chlamydia Trachomatis and Mycoplasma Genitalium
Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
متن کاملDevelopment and optimization of Multiplex- PCR for simultaneous detection of Porcine Pseudorabies Virus, Porcine Parvovirus, and Porcine Circovirus Type 2
Multiple infections by pathogens are currently the most serious problems in pig herds. Clinically, accurate diagnosis is difficult due to similarity of the symptoms of porcine pseudorabies virus (PRV), porcine parvovirus (PPV), and porcine circovirus type 2 (PCV2). A multiplex polymerase chain reaction (multiplex-PCR) was developed and optimized for the simultaneous detection of the three DNA v...
متن کاملDevelopment of real-time PCR assay for detection of porcine circovirus-like virus P1 in domestic pigs in China
BACKGROUND The porcine circovirus-like agent P1 is a newly discovered DNA virus with a single-stranded circular genome that is highly homologous to that of porcine circovirus type 2. P1 infection can cause symptoms resembling postweaning multisystemic wasting syndrome. This study aims to develop a rapid, sensitive and specific method to detect P1. RESULTS A pair of primers was designed and us...
متن کاملDevelopment and validation of a SYBR green real-time PCR for the quantification of porcine circovirus
not available 687 Krakowka, S., Ringler, S.S., Arumugam, P. , McKillen, J., McIntosh, K., Hartunian, C., Hamberg, A., Rings, M., Allan, G., Ellis, J.A. (2008) Evaluation of Mycoplasma hyopneumoniae bacterins for porcine torque teno virus DNAs American Journal of Veterinary Research, 69, 1601-1607 Objective-TO determine whether commercial Mycoplasma hyopneumoniae bacterins sold for use in swine ...
متن کاملPorcine Circovirus PCV-2
Porcine circovirus type 2 (PCV2) is considered by most to be the etiologic agent of postweaning multisystemic wasting syndrome (PMWS), but it is also found in association with numerous other conditions. These conditions include porcine dermatitis and nephropathy syndrome (PDNS), porcine respiratory disease complex (PRDC), congenital tremors (CT) type AII, reproductive failure and enteritis. Alt...
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ژورنال
عنوان ژورنال: Virologica Sinica
سال: 2018
ISSN: 1674-0769,1995-820X
DOI: 10.1007/s12250-018-0025-2